pTargeT (linearized) Sequence and Map (2024)

Linearized TA cloning vector with 3'-T overhangs for expressing PCR-amplified genes in mammalian cells.

Sequence Author: Promega

Try SnapGene for Free

|Download SnapGene Viewer

Explore Over

2.7k

Plasmids:

TA and GC Cloning Vectors

|

More Plasmid Sets

No matches

HomePlasmidsTA and GC Cloning VectorspTargeT (linearized)

Show Static Map

BglII(4382)
1 site
AlwNI(3961)
1 site
Sticky ends from different AlwNI sites may not be compatible.
AhdI(3482)
1 site
The 1-base overhangs produced by AhdI may be hard to ligate. Sticky ends from different AhdI sites may not be compatible.
BpmI(3413)
1 site
Efficient cleavage requires at least two copies of the BpmI recognition sequence.
Sticky ends from different BpmI sites may not be compatible.After cleavage, BpmI can remain bound to DNA and alter its electrophoretic mobility.BpmI quickly loses activity at 37°C.
ScaI(3001)
1 site
XmnI(2882)
1 site
EcoO109I(2502)
1 site
Sticky ends from different EcoO109I sites may not be compatible.
PfoI(2443)
1 site
Sticky ends from different PfoI sites may not be compatible.
BstXI(2241)
1 site
Sticky ends from different BstXI sites may not be compatible.
BstBI(2202)
1 site
RsrII(2036)
1 site
Efficient cleavage requires at least two copies of the RsrII recognition sequence.
Sticky ends from different RsrII sites may not be compatible.For full activity, add fresh DTT.
BssHII(1917)
1 site
BssHII is typically used at 50°C, but is 75% active at 37°C.
PflFI(1638)
1 site
The 1-base overhangs produced by PflFI may be hard to ligate.Sticky ends from different PflFI sites may not be compatible.
Tth111I(1638)
1 site
The 1-base overhangs produced by Tth111I may be hard to ligate.Sticky ends from different Tth111I sites may not be compatible.
BbeI(1523)
1 site
Cleavage may be enhanced when more than one copy of the BbeI recognition sequence is present.
SfoI(1521)
1 site
NarI(1520)
1 site
Efficient cleavage requires at least two copies of the NarI recognition sequence.
KasI(1519)
1 site
BsrGI(4483)
1 site
BsrGI is typically used at 37°C, but is even more active at 60°C.
SpeI(4539)
1 site
NdeI(4774)
1 site
Prolonged incubation with NdeI may lead to removal of additional nucleotides.
SnaBI(4880)
1 site
AsiSI(5051)
1 site
Eco53kI(5114)
1 site
SacI(5116)
1 site
BmgBI(5536)
1 site
This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.
AanI(5615)
3 sites
EcoRI(5637)
2 sites
BamHI(5643)
1 site
After cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.
NheI(5651)
1 site
BmtI(5655)
1 site
PaeR7I(5657)
1 site
PaeR7I does not recognize the sequence CTCTCGAG.
XhoI(5657)
1 site
AflIII(5663)
1 site
Sticky ends from different AflIII sites may not be compatible.
MluI(5663)
1 site
End(5672)
0 sites
Start(1)
0 sites
TspMI(8)
1 site
XmaI(8)
1 site
Cleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.
SmaI(10)
1 site
SmaI can be used at 37°C for brief incubations.
Acc65I(14)
1 site
KpnI(18)
1 site
SalI(20)
1 site
AccI(21)
1 site
Efficient cleavage with AccI requires ≥13 bp on each side of the recognition sequence.Sticky ends from different AccI sites may not be compatible.
NotI(28)
1 site
EcoRI(35)
2 sites
AanI(361)
3 sites
HpaI(381)
1 site
MfeI(390)
1 site
BsaBI(483)
1 site
* BlockedbyDammethylation.
BspDI(487)
1 site
* BlockedbyDammethylation.
ClaI(487)
1 site
* BlockedbyDammethylation.
BspEI(492)
1 site
PmlI(566)
1 site
DraIII(747)
1 site
Sticky ends from different DraIII sites may not be compatible.
AanI(872)
3 sites
SexAI(1093)
1 site
* BlockedbyDcmmethylation.Sticky ends from different SexAI sites may not be compatible.
SfiI(1279)
1 site
Efficient cleavage requires at least two copies of the SfiI recognition sequence.
Sticky ends from different SfiI sites may not be compatible.
BseRI(1322)
1 site
Sticky ends from different BseRI sites may not be compatible.BseRI quickly loses activity at 37°C.Prolonged incubation with BseRI may lead to degradation of the DNA.
StuI(1325)
1 site
AvrII(1326)
1 site

pTargeT™ Sequencing Primer
24-mer/38% GC
1 binding site
61 .. 84=24 annealed bases
Tm=55°C

AmpR
2695..3555=861bp
286 amino acids=31.6 kDa
2 segments
Segment 1:signal sequence
2695..2763=69bp
23 amino acids=2.6 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR
2695..3555=861bp
286 amino acids=31.6 kDa
2 segments
Segment 2:
2764..3555=792bp
263 amino acids=28.9 kDa
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics

AmpR
2695..3555=861bp
286 amino acids=31.6 kDa
2 segments
Product: β-lactamase
confers resistance to ampicillin, carbenicillin, and related antibiotics

NeoR/KanR
1392..2186=795bp
264 amino acids=29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)

NeoR/KanR
1392..2186=795bp
264 amino acids=29.0 kDa
Product: aminoglycoside phosphotransferase from Tn5
confers resistance to neomycin, kanamycin, and G418 (Geneticin)

ori
3726..4314=589bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

ori
3726..4314=589bp
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

f1 ori
515..969=455bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

f1 ori
515..969=455bp
f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis

SV40 promoter
984..1341=358bp
SV40 enhancer and early promoter

SV40 promoter
984..1341=358bp
SV40 enhancer and early promoter

CMV enhancer
4600..4904=305bp
human cytomegalovirus immediate early enhancer

CMV enhancer
4600..4904=305bp
human cytomegalovirus immediate early enhancer

lacZα
5438..5671=234bp
77 amino acids=9.0 kDa
Product: LacZα fragment of β-galactosidase

lacZα
5438..5671=234bp
77 amino acids=9.0 kDa
Product: LacZα fragment of β-galactosidase

CMV promoter
4905..5116=212bp
human cytomegalovirus (CMV) immediate early promoter

CMV promoter
4905..5116=212bp
human cytomegalovirus (CMV) immediate early promoter

chimeric intron
5277..5409=133bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes

chimeric intron
5277..5409=133bp
chimera between introns from human β-globin and immunoglobulin heavy chain genes

SV40 poly(A) signal
260..381=122bp
SV40 polyadenylation signal

SV40 poly(A) signal
260..381=122bp
SV40 polyadenylation signal

AmpR promoter
2590..2694=105bp

AmpR promoter
2590..2694=105bp

lacZα
2..94=93bp
31 amino acids=3.3 kDa
Product: LacZα fragment of β-galactosidase

lacZα
2..94=93bp
31 amino acids=3.3 kDa
Product: LacZα fragment of β-galactosidase

poly(A) signal
2250..2298=49bp
synthetic polyadenylation signal

poly(A) signal
2250..2298=49bp
synthetic polyadenylation signal

lac promoter
138..168=31bp
3 segments
Segment 3:-10
138..144=7bp
promoter for the E. coli lac operon

lac promoter
138..168=31bp
3 segments
Segment 2:
145..162=18bp
promoter for the E. coli lac operon

lac promoter
138..168=31bp
3 segments
Segment 1:-35
163..168=6bp
promoter for the E. coli lac operon

lac promoter
138..168=31bp
3 segments
promoter for the E. coli lac operon

lac operator
114..130=17bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

lac operator
114..130=17bp
The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).

SV40 ori
1192..1327=136bp
SV40 origin of replication

SV40 ori
1192..1327=136bp
SV40 origin of replication

MCS
2..40=39bp
multiple cloning site

MCS
2..40=39bp
multiple cloning site

MCS
5637..5671=35bp
multiple cloning site

MCS
5637..5671=35bp
multiple cloning site

T7 promoter
5616..5634=19bp
promoter for bacteriophage T7 RNA polymerase

T7 promoter
5616..5634=19bp
promoter for bacteriophage T7 RNA polymerase

ORF:1564 .. 1950=387 bp
ORF:128 amino acids=14.6 kDa

ORF:2695 .. 3555=861 bp
ORF:286 amino acids=31.6 kDa

ORF:1392 .. 2186=795 bp
ORF:264 amino acids=29.0 kDa

ORF:1701 .. 2237=537 bp
ORF:178 amino acids=19.8 kDa

ORF:3159 .. 3425=267 bp
ORF:88 amino acids=9.2 kDa

ORF:5438 .. 5671=234 bp
ORF:77 amino acids=9.0 kDa(no start codon)

Click here to try SnapGene

Download pTargeT (linearized).dna file

pTargeT (linearized) Sequence and Map (1)SnapGene

SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures

  • Fast accurate construct design for all major molecular cloning techniques
  • Validate sequenced constructs using powerful alignment tools
  • Customize plasmid maps with flexible annotation and visualization controls
  • Automatically generate a rich graphical history of every edit and procedure

Download SnapGene

pTargeT (linearized) Sequence and Map (2)SnapGene Viewer

SnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files.

  • Gain unparalleled visibility of your plasmids, DNA and protein sequences
  • Annotate features on your plasmids using the curated feature database
  • Store, search, and share your sequences, files and maps

Download SnapGene Viewer

Individual Sequences & Maps

Allele TA Vector (linearized)

pCR2.1-TOPO (linearized)

pJAZZ-OK GC (left arm)

pSpark TA

pBAD-TOPO (linearized)

pCR4-TOPO (linearized)

pJAZZ-OK GC (right arm)

pSpark TA Done

pBAD Thio-TOPO (linearized)

pCR8 GW TOPO (linearized)

pLenti6.3 V5-TOPO (linearized)

pBlue-TOPO (linearized)

pCRII-TOPO (linearized)

pLenti7.3 V5-TOPO (linearized)

pT7Blue T-Vector

pcDNA3.1 CT-GFP-TOPO (linearized)

pDrive (linearized)

pLPS-T (linearized)

pTA Plus (linearized)

pcDNA3.1 NT-GFP-TOPO (linearized)

pEF6 V5-His-TOPO (linearized)

pLUG (linearized)

pTargeT (linearized)

pcDNA3.1 V5-His-TOPO (linearized)

pENTR5'-TOPO (linearized)

pLUG-Multi (linearized)

pTOP TA V2 (linearized)

pcDNA3.3-TOPO (linearized)

pET SUMO (linearized)

pMT V5-His-TOPO (linearized)

pTrcHis-TOPO (linearized)

pcDNA3.4-TOPO (linearized)

pETBlue-1 AccepTor

pOptiVEC-TOPO (linearized)

pTrcHis2-TOPO (linearized)

pcDNA4 HisMax-TOPO (linearized)

pEXP5-CT TOPO (linearized)

pPrime (linearized)

pTZ57R_T

pcDNA5 FRT TO-TOPO (linearized)

pEXP5-NT TOPO (linearized)

pQE-30 UA (linearized)

pUC57-T

pcDNA5 FRT V5-His-TOPO (linearized)

pGC Blue (linearized)

pSC-A-amp kan (linearized)

pYES2.1 V5-His-TOPO (linearized)

pcDNA6.2 C-EmGFP-GW TOPO (linearized)

pGEM-T (linearized)

pSecTag FRT V5-His-TOPO (linearized)

pcDNA6.2 C-YFP-GW TOPO (linearized)

pGEM-T Easy (linearized)

pSMART GC HK (linearized)

T-Vector pMD19 (Simple)

pcDNA6.2 N-EmGFP-GW TOPO (linearized)

pGlow-TOPO (linearized)

pSMART GC LK (linearized)

T-Vector pMD20

pCR-XL-TOPO (linearized)

pIB V5-His-TOPO (linearized)

Plasmid Sets

Basic Cloning Vectors

I.M.A.G.E. Consortium Plasmids

TA and GC Cloning Vectors

Coronavirus Resources

Insect Cell Vectors

pGEX Vectors (GE Healthcare)

TOPO Cloning Vectors

CRISPR Plasmids

Luciferase Vectors

Plant Vectors

Lucigen Vectors

Qiagen Vectors

Yeast Plasmids

Gateway® Cloning Vectors

Mammalian Expression Vectors

Structural Genomics Vectors

The maps, notes, and annotations in the zip file on this page are copyrighted material. This material may be used without restriction by academic, nonprofit, and governmental entities, except that the source must be cited as ’’www.snapgene.com/resources’’. Commercial entities must contact GSL Biotech LLC for permission and terms of use.

Discover the most user-friendly molecular biology experience.

Try for Free

pTargeT (linearized) Sequence and Map (2024)

References

Top Articles
Latest Posts
Article information

Author: Zonia Mosciski DO

Last Updated:

Views: 6458

Rating: 4 / 5 (71 voted)

Reviews: 94% of readers found this page helpful

Author information

Name: Zonia Mosciski DO

Birthday: 1996-05-16

Address: Suite 228 919 Deana Ford, Lake Meridithberg, NE 60017-4257

Phone: +2613987384138

Job: Chief Retail Officer

Hobby: Tai chi, Dowsing, Poi, Letterboxing, Watching movies, Video gaming, Singing

Introduction: My name is Zonia Mosciski DO, I am a enchanting, joyous, lovely, successful, hilarious, tender, outstanding person who loves writing and wants to share my knowledge and understanding with you.